Details

Project TitlePREVASCULARIZED DEVICES AND RELATED METHODS (16089)
Track Code16089
Short Description
Abstract

Particularized Devices and Related Methods

Features and Benefits

  • May be used for the formation of adipose derived regenerative cell laiden spheroids of type I collagen for translational use
  • Avoids the inhibition of cellular functions as demonstrated by other hydrogels such as alginate
  • Spheroids can be produced and implanted immediately
  • May incorporate many other cell types

 *This Technology is available for licensing, further development, or industrial partnering*


Market Opportunities

Current methods of translational cell-based angiogenesis require in vitro 2D tissue culture wherein cells are grown selectively in monolayer and isolated thereafter for in vivo or clinical models.  However, using 3D bioprinting technology with stromal vascular fraction (SVF) cells isolated from adipose tissue, spheroids of type I collagen laiden with SVF can be engineered rapidly and in an automated fashion for immediate implantation. Also, this new method requires no extra additives to the culture that could inhibit cellular functions and utilizes natural extracellular matrix (collagen I) as the microenvironment to support cell proliferation. Already this novel method has been effective in creating spheroids that can maintain shape, support cell viability over 14 days, as well as promote vasculogenesis and angiogenesis in vitro. This method of spheroid production has the capability to be effective in drug screening of potential inhibitors of angiogenesis or vasculogenesis. SVF laiden spheroids provide a unique translational approach where patient specific SVF can be isolated, bioprinted and reinjected to mitigate ischemic disease states such as peripheral vascular disease or acute myocardial infarction, thus providing the nidus for microvascular reorganization. These tissue-engineered constructs may provide insight into cell-based therapies that can integrate with host vasculature, support reperfusion to diseased tissues, and provide a microenvironment allowing vascular network sustainability prior to inosculation.

     

Technology  

Stromal vascular fraction contains multiple cell types that can self-assemble into microvasculature both in vitro and in vivo. These cell types include endothelial cells, smooth muscle cells, pericytes, tissue resident immune cells as well as circulating and tissue resident progenitor and stem cells including mesenchymal stem cells. These cells can communicate with one another in tissue culture to self-assemble into microvascular structures that can be transplanted allowing for inosculation and reperfusion of both tissue-engineered vessels and endogenous vessels. Previous methods to create cell-laiden spheroids utilized alginate, a chitosan derived from plant algae as a hydrogel to support 3D construct formation. The gel could then be printed into small spheroids containing SVF, however, the use of alginate provided a microenvironment with reduced cellular motility and proliferation.  Cells were viable but could not undergo molecular signaling  allowing for the morphogenesis into microvessel-like structures in vitro. This new method uses a 3D printer to print a solution of type I collagen containing SVF that can polymerize into 3D spheroids. By utilizing a super-hydrophobic surface, the printed spheroid in liquid form can polymerize overtime while still maintaining a sphere shape. These spheroids, have been shown to support viability of the cells as well as the promote formation of neovasculature in vitro.

Technology Status

  • IP Status: Patent pending
  • Fields of Use Available: All
  • Development Status: Further development needed

    

Researchers:

  • Stuart Williams

  • Brian Gettler

  • Joseph Zakhari

  • Piyani Gandhi

 
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Posted DateMay 12, 2017 11:34 AM